Is It Possible to Test by a Direct Experiment the Hypothesis by Brillouin L.

By Brillouin L.

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5-mL aliquots of cells to five cryovials, approx. 5 × 106 cells per vial. Keep caps of cryovials with internal thread sterile by standing them on the inside of the 15-cm plate lid. For ES cells, aspirate media, resuspend in 2 mL solution I, then add 2 mL solution II and dispense 1-mL aliquots into four cryovials, approx. 2 × 106 cells per vial. 3. Place cryovials into a polystyrene 15-mL centrifuge tube rack “sandwich” and place at –70°C to –80°C overnight. Alternatively, use commercially available containers designed to cool at a more controlled rate.

Zygotes formed from gametes with complementary duplications and deficiencies of most chromosome regions usually give rise to normal, viable mice. If an imprinted chromosomal region is involved in the translocation, the perturbed parental origin of the region can cause developmental defects associated with the abnormal dosage of the imprinted gene or genes. A catalog of chromosomal variants, including reciprocal and Robertsonian translocations and some information on their origin, fertility, and frequency of nondisjunction, has been documented by Beechey and Evans (9).

Kono, T. and Tsunoda, Y. (1988) Nuclear transplantation of rat embryos. J. Exp. Zool. 248, 303–305. 28. , Diaz, E. , Prideaux, V. , and Rossant, J. (1990) Embryonic stem cells alone are able to support fetal development in the mouse. Development 110, 815–821. 29. McLaughlin, K. J. (1993) Production of tetraploid embryos by electrofusion. Methods Enzymol. 225, 919–930. 30. Lawitts, J. A. and Biggers, J. D. (1993) Culture of preimplantation embryos. Methods Enzymol. 225, 153–190. 31. , and Lacy, E.

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