Human Cell Culture Protocols by Joanna Picot

By Joanna Picot

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Smith, A. , and Zarek, J. M. (1972) Isolation of living cells from mature mammalian bone. Nature 236, 314–315. 3. 3 Mills, B. , Singer, F. , Weiner, L. , and Hoist, P. A. (1979) Long term culture of cells from bone affected with Paget’s disease. Calcif. Tiss. Int. 29, 79–87. 4. 4 Gallagher, J. , Beresford, J. , McGuire, M. K. , et al. (1984) Effects of glucocorticoids and anabolic steroids on cells derived from human skeletal and articular tissues in vitro. Adv. Exp. Med. Biol. 171, 279–292. 5.

5–4 × 106 cells/75-cm2 flask after 28 d in primary culture. Cell viability is generally ≥90%. 2. Osteoblastic Cell Lines Osteosarcomas are malignant tumors of bone derived from cells of the osteoblast lineage. An osteosarcoma is a relatively uncommon tumor, although it is the most frequently occurring primary malignant tumor of bone, predominantly occurring in children and adolescents. It is also reported to occasionally occur in elderly patients with long-standing Paget’s disease of bone. Histologically, the tumor is heterogeneous, the cells being associated with immature bone matrix or osteoid.

Invest. 110, 751–759. 54 Gartland et al. 49. Mason, J. , Breitbart, A. , Pergolizzi, R. , and Grande, D. A. (2000) Cartilage and bone regeneration using gene-enhanced tissue engineering. Clin. Orthop. ), S171–S178. 50. 50 Gehron-Robey, P. and Termine, J. D. (1985) Human bone cells in vitro. Calcif. Tiss. Int. 37, 453, 460. 51. 51 Wergedal, J. E. and Baylink, D. J. (1984) Characterisation of cells isolated and cultured from human trabecular bone. Proc. Soc. Exp. Biol. Med. 176, 60–69. 52. Marie, P.

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