Corona- and Related Viruses: Current Concepts in Molecular by Ellen Buschman, Emil Skamene (auth.), Pierre J. Talbot, Gary

By Ellen Buschman, Emil Skamene (auth.), Pierre J. Talbot, Gary A. Levy (eds.)

Corona- and comparable viruses are vital human and animal pathogens that still function types for different viral-mediated ailments. curiosity in those pathogens has grown vastly because the First overseas Symposium used to be held on the Institute of Virology and Immunobiology of the collage of Wiirzburg, Germany. The 6th overseas Symposium used to be held in Quebec urban from August 27 to September I, 1994, and supplied extra realizing of the molecular biology, immunology, and pathogenesis of corona-, toro-, and arterivirus infections. Lectures got at the molecular biology, pathogenesis, immune responses, and improvement of vaccines. stories at the pathogenesis of coronavirus infections were centred frequently on murine coronavirus, and mouse hepatitis virus. Neurotropic lines ofMHV (e.g., JHM, A59) reason a demyelinating sickness that has served as an animal version for human a number of sclerosis. Dr. Samuel Dales, of the collage of Western Ontario, London, Canada, gave a cutting-edge lecture on our present less than­ status of the pathogenesis of JHM-induced disease.

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The ISU-l (Ind/89) and ISU-3 (NC/89) strains of PRCV provided by Dr. H. , Ames, Iowa24 were plaque-purified twice and passaged 8-14 times in ST cells. These viruses produce only subclinical infections when inoculated into gnotobiotic or conventional pigs and replicate exclusively in the upper respiratory tract21 , 25. The PRCV strain designated DD312 was recently isolated from the respiratory tract of a pig in the United States (Saif, Weilnau and Gadfield, unpublished). Monoclonal Antibodies (MAbs) TGEV-specific MAbs were produced and characterized in our laboratory 12· 13, 14.

Cleavage patterns for the MHV-Y clones differed substantially from those predicted from MHV-A59 and MHV-4/JHM S sequences, so the entire MHV-Y S gene was sequenced 13-15. en LI'l

2070) behaved also as highly fusogenic strains. Changes in the amino acid sequences of the S glycoproteins between these two strains and the reference BCV-Meb strain were recently reported l5 . Whether these differences at the molecular level could be related to the BCV virulence remains to be elucidated. Our results could also differentiate between WD and NCD strains on basis of their interaction with different erythrocytes. Both NCD and WD isolates agglutinated rat erythrocytes with similar titers at 4°C, but a drastic drop was noticed in the HA titers of WD isolates at 37°C.

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