Cell Fusion: Overviews and Methods by Casey A. Ydenberg, Mark D. Rose (auth.), Elizabeth H. Chen

By Casey A. Ydenberg, Mark D. Rose (auth.), Elizabeth H. Chen (eds.)

Exciting paintings long ago decade has printed commonalities and ameliorations between person mobilephone fusion occasions. In Cell Fusion: Overviews and Methods, a staff of top specialists offer a suite of overviews that define our present figuring out of mobile fusion and strategies that current vintage and cutting-edge experimental ways in quite a few platforms. Divided into handy components, the quantity starts with 9 overviews which describe various phone fusion occasions in types from yeast to mammals, and it maintains with 13 chapters illustrating favourite how you can assay telephone fusion specifically structures. As part of the hugely profitable Methods in Molecular Biology™ series, those equipment chapters collect step by step, quite simply reproducible protocols with lists of the required fabrics and reagents, in addition to pointers on troubleshooting and keeping off identified pitfalls.

Cutting-edge and effortless, Cell Fusion: Overviews and Methods serves as a complete source for a person, specialist or beginner, drawn to the attention-grabbing organic strategy of cellphone fusion.

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2006) Nonself recognition and programmed cell death in filamentous fungi. Curr. Opin. Microbiol. 9, 553–558. 65. Glass, N. L. and Kaneko, I. (2003) Fatal attraction: nonself recognition and heterokaryon incompatibility in filamentous fungi. Eukaryot. Cell 2, 1–8. 66. Saupe, S. J. (2000) Molecular genetics of heterokaryon incompatibility in filamentous ascomycetes. Microbiol. Mol. Biol. Rev. 64, 489–502. 67. , Townsend, J. , Gilbert, L. , Taylor, J. , and Glass, N. L. (2005) Long-oligomer microarray profiling in Neurospora crassa reveals the transcriptional program underlying biochemical and physiological events of conidial germination.

14. Potgens, A. J. , and Frank, H. G. (2002) Mechanisms of syncytial fusion: a review. Placenta 23 (Suppl A, Trophoblast Res. 16), 107–113. 15. Davis, R. H. (2000) Neurospora: Contributions of a Model Organism. Oxford University Press, New York. 16. Perkins, D. , and Sachs, M. S. (2001) The Neurospora Compendium: Chromosomal Loci. Academic Press, San Diego. 17. Colot, H. , Turner, G. , Crew, C. , Weiss, R. , Borkovich, K. , and Dunlap J. C. (2006) A high-throughput gene knockout procedure for Neurospora reveals functions for multiple transcription factors.

Germinating pollen tubes are also thought to be guided by diffusible chemotropic substances, such as Ca2+ or small heatstable molecules secreted by the style (29,30). Another diffusible substance that is released by synergid cells guides the pollen tube into the ovule (31). The eggs of many aquatic animal species also release chemotactic substances to attract sperm; for example, Xenopus egg jelly releases a cysteine-rich secretory protein, allurin, to attract sperm (32). In these examples, the fusion partners are genetically and/or physiologically distinct and either secrete different signaling molecules (such as mating-type–specific pheromones) or only one partner secretes a signal that results in the attraction of the other partner.

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