Carbon Nanotubes: Methods and Protocols by Luís F. F. Neves, Ta-Wei Tsai, Naveen R. Palwai, David E.
By Luís F. F. Neves, Ta-Wei Tsai, Naveen R. Palwai, David E. Martyn, Yongqiang Tan (auth.), Kannan Balasubramanian, Marko Burghard (eds.)
Due to their infrequent mix of excessive chemical balance, extraordinary optical and electric homes, excessive surface-to-volume ratio, and excessive point ratio, carbon nanotubes (CNTs) have made an incredible influence on fabrics technological know-how, molecular biology, biomedicine, and bioanalytical chemistry. Carbon Nanotubes: tools and Protocols presents trustworthy, constant protocols at the program of CNTs in molecular biology-related fields. those are of important significance, because the commercially to be had CNTs range in purity, agglomeration nation, in addition to size and diameter distribution, all of that have a profound impact at the dispersability and floor homes of the tubes. the quantity comprises exact sections on functionalization, toxicity, trafficking, scaffolds, and biosensors, supplied by means of professional researchers from a number of fields. Written within the hugely winning Methods in Molecular Biology™ sequence structure, chapters comprise introductions to their respective themes, lists of the mandatory fabrics and reagents, step by step, with no trouble reproducible laboratory protocols, and notes on troubleshooting and averting identified pitfalls.
Authoritative and state-of-the-art, Carbon Nanotubes: tools and Protocols serves to give a contribution to the fulfillment of universal criteria and is helping researchers to prevent discrepancies in destiny biology-related CNT studies.
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Extra info for Carbon Nanotubes: Methods and Protocols
7002100) are gratefully acknowledged. References 1. Iijima S (1991) Helical microtubules of graphitic carbon. Nature 354:56–58 2. Ebbesen TW, Ajayan PM (1992) Large-scale synthesis of carbon nanotubes. Nature 358: 220–222 3. Chen RJ, Zhang YG, Wang DW, Dai HJ (2001) Noncovalent sidewall functionalization of single-walled carbon nanotubes for protein immobilization. J Am Chem Soc 123:3838–3839 4. Heller DA, Baik S, Eurell TE, Strano MS (2005) Single-walled carbon nanotube spectroscopy in live cells: towards long-term labels and optical sensors.
Carbon nanotubes appear as bright lines in the image. 3b, DNA strands are connected to the sidewall of nanotubes, indicating that oxidization took place in the defect sites of sidewalls. From the observations of several samples, we conclude that DNA attachment occurred at nanotube ends and sidewalls, because chemical functionalization occurred primarily at the ends and sidewalls. 4 Notes 1. I water. 2. I water and followed by ultrasonication for 30 min. 3. Use only polyethylene gloves. Other gloves may contain silicones that can contaminate the surface.
Kam NWS, O’Connell M, Wisdom JA, Dai H (2005) Carbon nanotubes as multifunctional biological transporters and nearinfrared agents for selective cancer cell destruction. Proc Natl Acad Sci USA 102:11600–11605 23. Kam NWS, Liu Z, Dai H (2006) Carbon nanotubes as intracellular transporters for proteins and DNA: an investigation of the uptake mechanism and pathway. Angew Chem Int Ed Engl 45:577–581 24. Barone PW, Strano MS (2006) Reversible control of carbon nanotube aggregation for a glucose affinity sensor.