Biological Response to Cement containing Acrylic Polymers

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1 Schematic diagram of a transmission electron microscope. Adapted from a drawing provided by Roger C. htm). 1. 1 Electrons are strongly scattered 1 This section is not meant as a substitute for a systematic introduction, but serves mainly to provide a general orientation, and to introduce basic terms that will be used in the text. 15 16 THREE-DIMENSIONAL ELECTRON MICROSCOPY by matter. , 1996). Values for resins used for plastic embedding and for frozen-hydrated tissue closely match those for vitreous ice.

The double-carbon layer method of staining [Valentine et al. (1968); Tischendorf et al. (1974); Sto00 ffler and Sto00 ffler-Meilicke (1983); see also Frank et al. (1986), where the relative merits of this technique as compared to those of the single-layer technique are discussed] solves the problem of partial stain immersion ELECTRON MICROSCOPY OF MACROMOLECULAR ASSEMBLIES 25 by providing staining of the particle from both sides without information loss. , 1988) precisely for that reason: antibodies attached to the surface facing away from the primary carbon layer will then be visualized with the same contrast as those close to the carbon film.

Outlines of the particles are better defined in the micrograph because a stain meniscus forms around the particle border (see Moody, 1967), producing a sharp increase in scattering absorption there. In contrast to this behavior, the stain is confined to a wedge at the particle border in sandwich preparations, producing a broader, much more uniform band of stain. Intuitively, the electron microscopist is bound to prefer the appearance of particles that are sharply delineated, but the computer analysis shows that images of sandwiched particles are in fact richer in interior features.

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