Azospirillum IV: Genetics · Physiology · Ecology Proceedings by M. Galimand, C. Vieille, B. Perroud, I. Onyeocha, C.
By M. Galimand, C. Vieille, B. Perroud, I. Onyeocha, C. Elmerich (auth.), Prof. Dr. Walter Klingmüller (eds.)
th th On June 17 and 18 1987 the fourth workshop on "Azospi rillum: Genetics, body structure, Ecology" happened on the Uni ver sity of Bayreuth, West Germany, prepared by means of the Genetics go away ment. there have been approximately eighty individuals, who got here from German study associations, from different eu international locations, from Is rael, Egypt, India, North Vietnam, and North and South the United States. the previous workshops had additionally taken position in Bayreuth, in 1981, 1983 and 1985, therefore the organizers may perhaps draw at the event got at those prior workshops. Azospirilla have, in the past 15 years, came upon an ever expanding medical curiosity simply because, first, those soil bacte ria hold the genetic info for binding molecular nitrogen from the air and, moment, they stay in shut area to the roots of grain vegetation and forage grasses. by way of exploitation of those homes, it truly is was hoping to advance inoculation systems which might be utilized in nitrogen-deficient soils.
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Additional resources for Azospirillum IV: Genetics · Physiology · Ecology Proceedings of the Fourth Bayreuth Azospirillum Workshop
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Plasmid DNA was prepared by the cleared using Triton X-IOO. Bacteriophages trifugation on a CsCl step gradient described by Maniatis et al. lysate were purified and the DNA (8). method by cenisolated as DNA was digested withre- striction endonucleases according to the instructions of the suppliers. The DNAfragments were separated by eletrophoresis on agarose gels and then transferred to nitrocellulose or nylon membranes (9). Hybridizations were performed as described using 32p-labelled probes. (8) 5.
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