Atomic Force Microscopy: Biomedical Methods and Applications by Davide Ricci, Pier Carlo Braga

By Davide Ricci, Pier Carlo Braga

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Typically, the artifacts will appear as oscillations. Both acoustic and floor vibrations can excite vibrational modes in an AFM and cause artifacts. The floor in a building can vibrate vertically several micrometers at frequencies below 5 Hz. The floor vibrations, if not properly filtered, can cause periodic structure in an image. This type of artifact is most often noticed when imaging very flat samples. Sometimes the vibrations can be started by an external event such as an elevator in motion, a train going by, or even people walking in a hallway.

Et al. (2000) Stress at the solid-liquid interface of self-assembled monolayers on gold investigated with a nanomechanical sensor. Langmuir 16, 9694–9696. 9. Battiston F. , Ramseyer J. , Lang H. , et al. (2001) A chemical sensor based on a microfabricated cantilever array with simultaneous resonance-frequency and bending readout. Sensors Actuators B 77, 122–131. 10. , and Semenza, G. (1996) Nhydroxysuccinimide ester functionalized self-assembled monolayers for covalent immobilization of biomolecules on gold.

Microsc. Res. Tech. 44, 312–326. 10. Dinte, B. , Watson, G. , Dobson, J. , and Myhra, S. (1996) Artefacts in noncontact mode force microscopy: The role of adsorbed moisture. Ultramicroscopy 63, 115–124. 11. , and Shao, Z. (1996) The effect of deformation on the lateral resolution of the atomic force microscopy. J. Microsc. 182, 106–113. 12. van Noort, S. , van der Werf, K. , de Grooth, B. , van Hulst, N. , and Greve, J. (1997) Height anomalies in tapping mode atomic force microscopy in air caused by adhesion.

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