Applications of Continuous and Steady-State Methods to Root by D. B. Layzell, S. Hunt, B. J. King (auth.), John G. Torrey,

By D. B. Layzell, S. Hunt, B. J. King (auth.), John G. Torrey, Lawrence J. Winship (eds.)

This ebook offers an outstanding representation of the interrelationship among growth in medical technique and conceptual advances, and its publica­ tion may still give a contribution to additional advances. it really is popular that significant advances in realizing usually persist with the advance of recent tools. the improvement of the acetylene aid assay for nitrogenase task offers a great instance of this interrelationship among thought and techniques. Theoretical wisdom ended in a look for substrates for nitro­ genase which may be assayed for extra simply than ammonium, the conventional made from the enzyme. the invention of the aid of acetylene to ethylene by means of nitrogenase supplied the best resolution to the matter by means of provid­ ing a fast, particular, nondestructive, and cheap assay for nitrogenase task. This assay is now utilized by virtually each laboratory doing learn on nitrogen fixation. although, extra use and improvement of the acetylene relief assay has proven that it may underestimate nitrogenase job and will even supply improper relative values below a few conditions. the key challenge is that publicity of legume nodules to acetylene could cause a wide bring up within the resistance to oxygen diffusion into the nodule. This lowered offer of oxygen decreases the speed of nitrogenase task inside a number of minutes.

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For example, in a recent report (Walsh et al. 1987) the effect of photoperiod on the rates of CO 2 and Hz exchange by nodulated roots of soybean over a 4-day period was examined. In other studies, the effects of low root temperature on nitrogenase activity (Layzell et al. 1984a) and C and N partitioning (Walsh and Layzell 1986) were measured in soybean over periods of 4 to 11 days. In the latter study, the 12-channel system was used to monitor continuously the rates of CO 2 and H2 exchange from 8 nodulated roots and 2 shoot systems in plants with root temperatures held at either 25°C or 15 °C (Fig.

Witty JF, L Skat, NP Revsbech 1987 Direct evidence for changes in the resistance of legume root nodules to O 2 diffusion. J Exp Bot 38: 1129-1140. Chapter 2 Quantification of Diffusion Characteristics in Spherical Nodules: A Comparison of Methods P. R. SINCLAIR USDA-ARS, Agronomy Physiology Laboratory, Bldg. 164, University of Florida, Gainesville, FL 32611, USA 1. Introduction Nitrogenase, the enzyme responsible for nitrogen fixation, is inactivated by oxygen (Bergersen 1962), yet leguminous nitrogen fixation is a highly energydependent reaction.

J Exp Bot 38: 1129-1140. Chapter 2 Quantification of Diffusion Characteristics in Spherical Nodules: A Comparison of Methods P. R. SINCLAIR USDA-ARS, Agronomy Physiology Laboratory, Bldg. 164, University of Florida, Gainesville, FL 32611, USA 1. Introduction Nitrogenase, the enzyme responsible for nitrogen fixation, is inactivated by oxygen (Bergersen 1962), yet leguminous nitrogen fixation is a highly energydependent reaction. A high energy requirement necessitates high rates of oxygen consumption (Tjepkema 1971).

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