An Introduction to Genetic Engineering by Dr Desmond S. T. Nicholl

By Dr Desmond S. T. Nicholl

During this 3rd version of his well known undergraduate-level textbook, Des Nicholl recognises sound clutch of easy rules is essential in any creation to genetic engineering. consequently, in addition to being completely up to date, the ebook additionally keeps its concentrate on the elemental rules utilized in gene manipulation. The textual content is split into 3 sections: half I offers an creation to the appropriate uncomplicated molecular biology; half II, the tools used to control genes; and half III, purposes of the know-how. there's a new bankruptcy dedicated to the rising value of bioinformatics as a unique self-discipline. different extra beneficial properties comprise textual content packing containers, which spotlight vital features of subject matters mentioned, and bankruptcy summaries, which come with goals and studying results. those, besides key be aware listings, idea maps and a word list, will allow scholars to tailor their examine to fit their very own studying kinds and finally achieve a company clutch of a topic that scholars usually locate tricky.

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The sequence of the coding strand is usually reported when dealing with DNA sequence data, as this permits easy reference to the sequence of the RNA. In addition to the sequence of bases that specifies the codons in a protein-coding gene, there are other important regulatory sequences associated with genes (Fig. 6). A site for starting transcription is required, and this encompasses a region that binds RNA polymerase, known as the promoter (P), and a specific start point for transcription 16:16 P1: SBT 9780521850063c02 CUUS128/Nicholl 978 0 521 85006 3 March 11, 2008 INTRODUCING MOLECULAR BIOLOGY Fig.

3 The structure of a nucleotide. Carbon atoms are represented by solid circles, numbered 1 to 5 . In DNA the sugar is deoxyribose, with a hydrogen atom at position X. In RNA the sugar is ribose, which has a hydroxyl group at position X. The base can be A, G, C, or T in DNA, and A, G, C, or U in RNA. 16:16 17 P1: SBT 9780521850063c02 18 CUUS128/Nicholl 978 0 521 85006 3 March 11, 2008 THE BASIS OF GENETIC ENGINEERING Fig. 4 Base-pairing arrangements in DNA. (a) An A · T base pair. The bases are linked by two hydrogen bonds (dotted lines).

If the cake is not mixed or baked properly, even with the correct proportions of ingredients, it will not turn out to be a success. Genes provide the information to specify the proteins, but the whole process must be controlled and regulated if the cell is to function effectively. 2 Transcription and translation These two processes are the critical steps involved in producing functional proteins in the cell. Transcription involves synthesis of an RNA from the DNA template provided by the non-coding strand of the transcriptional unit in question.

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