Advances in Microbial Physiology, Vol. 20 by A. H. & Morris, J. Gareth [eds.] Rose

By A. H. & Morris, J. Gareth [eds.] Rose

This quantity in a research-level sequence covers various facets of microbial body structure and biochemistry together with inositol metabolisms in yeasts, bacterial adhesion, natural acids, the bacterial flagellum and the mechanical behaviour of bacterial mobilephone partitions. it really is meant to be of use to microbiologists, biochemists and biotechnologists. different comparable works during this sequence are volumes 29, 30 and 31.

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Advances in Microbial Physiology, Vol. 20

This quantity in a research-level sequence covers different points of microbial body structure and biochemistry together with inositol metabolisms in yeasts, bacterial adhesion, natural acids, the bacterial flagellum and the mechanical behaviour of bacterial mobilephone partitions. it really is meant to be of use to microbiologists, biochemists and biotechnologists.

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Cyanobactcrial rihosomal 44 W. FORD DOOLllTLE proteins have been resolved in one-dimensional electrophoretic systems at least twice (Vasconcelos and Bogorad, 1971; Yurina and Odintsova, 1974). They are different from ribosomal proteins of E. coli and chloroplasts and show little reactivity with antisera directed against E. coli ribosomes (Wittmann, 1970). , 1970; Leach and Carr, 1974; Gray and Herson, 1976). Heterologous hybrids made from E. coli 50s and A. nidulans 30s subunits (or the reverse) a r e also functional.

Ssymank et al. (1977) observed residual incorporation of label into density-gradient-purified DNA for at least eight hours after darkening; actual rates of synthesis could not be calculated because precursor pool specific activities were unknown (and were likely to have varied). When chromosomes resume replication after reillumination of darkened A . nidulans, they appear to d o so in genetic synchrony. Asato and Folsome ( 1970) measured the frequency of nitrosoguanidine-induced forward mutations at several rather loosely defined genetic loci after reillumination; they found sequential and more-or-less discrete stepwise increases in frequency, and ordered their markers in a “temporal genetic” map.

Both Asato and Folsome (1970) and Herdman et al. (1970) observed two to three cycles of synchronous cell-division and DNA synthesis in reilluminated cells, although the former found periods of DNA synthesis to coincide with cell division and the latter found these to alternate. It is also noteworthy that the DNA synthetic period lasted only 60 to 100 mins in the rapidly growing (two hour doubling time) cultures of Herdman et al. ( 1970) but approximately 400 to 500 mins in the slowly growing (eight to nine hour doubling time) cultures of Asato and Folsome (1970).

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